Coding
Part:BBa_K5306001:Design
Designed by: Iulia Beres Group: iGEM24_MSP-Maastricht (2024-09-26)
Design Considerations
This coding sequence for nasE has been obtained from the organism Klebsiella oxytoca M5al and has been codon optimized specifically for our chassise organism Vibrio natriegens using Benchlingand a personal codon optimization table that can be found on our wiki Codon Optimization Vibrio natriegensaccording to codon frequency taken from Lee et al. (2019).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 409
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 409
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 409
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 409
Illegal AgeI site found at 502 - 1000COMPATIBLE WITH RFC[1000]
References
- Lee, H. H., Ostrov, N., Wong, B. G., Gold, M. A., Khalil, A. S., & Church, G. M. (2019). Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Nature Microbiology, 4(7), 1105–1113. https://doi.org/10.1038/s41564-019-0423-8
- Benchling. (2023). Cloud-Based Informatics Platform for Life Sciences R&D. Benchling. https://www.benchling.com/