Coding

Part:BBa_K5306001:Design

Designed by: Iulia Beres   Group: iGEM24_MSP-Maastricht   (2024-09-26)

Design Considerations

This coding sequence for nasE has been obtained from the organism Klebsiella oxytoca M5al and has been codon optimized specifically for our chassise organism Vibrio natriegens using Benchlingand a personal codon optimization table that can be found on our wiki Codon Optimization Vibrio natriegensaccording to codon frequency taken from Lee et al. (2019).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 409
    Illegal AgeI site found at 502
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. Lee, H. H., Ostrov, N., Wong, B. G., Gold, M. A., Khalil, A. S., & Church, G. M. (2019). Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Nature Microbiology, 4(7), 1105–1113. https://doi.org/10.1038/s41564-019-0423-8
  2. Benchling. (2023). Cloud-Based Informatics Platform for Life Sciences R&D. Benchling. https://www.benchling.com/